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KMID : 0364820140500020158
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Korean Journal of Microbiology
2014 Volume.50 No. 2 p.158 ~ p.163
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Production and Properties of Mannanase by a Bacillus amyloliquefaciens Isolate
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Yoon Ki-Hong
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Abstract
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In the acidic LB plate, a bacterial strain was isolated from homemade soybean paste as a producer of the
extracellular mannanase. The isolate YB-1402, which was a Gram-positive rod-shaped bacterium with spore, has
been identified as Bacillus amyloliquefaciens on the basis of its 16S rDNA sequence and biochemical properties.
Maximum mannanase productivity of the isolate YB-1402 was reached approximately 150 U/ml in LB broth
supplemented with konjac (3.0%). The molecular mass of YB-1402 mannanase was estimated to approximately 38.0
kDa by zymogram of the culture filtrate on SDS-PAGE. The mannanase of culture filtrate was the most active at
55℃ and pH 5.5. The mannanase activity was completely maintained after pre-incubation at pH 3.0 to 10.0 for 1 h.
The predominant products resulting from the mannanase hydrolysis were mannose, mannobiose and mannotriose for
LBG or mannooligosaccharides. The enzyme could hydrolyze mannooligosaccharides larger than mannobiose.
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KEYWORD
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Bacillus amyloliquefaciens, mannanase, productivity, property
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